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یافته های نوین در علوم زیستی، جلد ۱۲، شماره ۲، صفحات ۰-۰
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| عنوان فارسی |
Somatic Embryogenesis and Histological Aspect of Galbanum (Ferula gummosa Boiss.) Endangered Medicinal Plant |
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| چکیده فارسی مقاله |
Ferula gummosa Boiss. as a valuable pharmaceutical and industrial plant grows in Iran. In order to minimize seed dormancy period and micropropagation, callus induction and embryogenesis were evaluated. The plantlets of the seeds were separated and cultured in the 1/2 MS medium. After 14 days, root, hypocotyl, cotyledon and leaf explants were separated. Then they were transferred to the basal MS medium containing different concentrations of growth regulators. Different developmental stages of somatic embryos were evaluated. Seedlings 2-3 days after locating in 1/2 MS medium germinated and whole plantlets were obtained after 12 days. In the callus induction phase growth regulator composition 1.5 mgl-1 of NAA and 0.5 mgl-1 of BA with root explants had proper results. In the somatic embryogenesis phase MS medium containing 0.5 mgl-1 of 2,4-D accompanied by 1 mgl-1 of BA lead to desirable results. Via taken sections from the embryos, different developmental stages of somatic embryos including pre-embryo, globular, heart shaped, torpedo and cotyledonary embryo were observed. In vitro culture of embryo to accelerate germination and elimination of long dormancy period and using this optimized method are strongly suggested for micropropagation of this plant so this valuable endemic plant may survive from extinction, too.
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Somatic Embryogenesis and Histological Aspect of Galbanum (Ferula gummosa Boiss.) Endangered Medicinal Plant |
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| چکیده انگلیسی مقاله |
Ferula gummosa Boiss. as a valuable pharmaceutical and industrial plant grows in Iran. In order to minimize seed dormancy period and micropropagation, callus induction and embryogenesis were evaluated. The plantlets of the seeds were separated and cultured in the 1/2 MS medium. After 14 days, root, hypocotyl, cotyledon and leaf explants were separated. Then they were transferred to the basal MS medium containing different concentrations of growth regulators. Different developmental stages of somatic embryos were evaluated. Seedlings 2-3 days after locating in 1/2 MS medium germinated and whole plantlets were obtained after 12 days. In the callus induction phase growth regulator composition 1.5 mgl-1 of NAA and 0.5 mgl-1 of BA with root explants had proper results. In the somatic embryogenesis phase MS medium containing 0.5 mgl-1 of 2,4-D accompanied by 1 mgl-1 of BA lead to desirable results. Via taken sections from the embryos, different developmental stages of somatic embryos including pre-embryo, globular, heart shaped, torpedo and cotyledonary embryo were observed. In vitro culture of embryo to accelerate germination and elimination of long dormancy period and using this optimized method are strongly suggested for micropropagation of this plant so this valuable endemic plant may survive from extinction, too.
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| کلیدواژههای انگلیسی مقاله |
Callus Formation,Embryogenesis Stages,Growth Regulators,Root Explants |
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| نویسندگان مقاله |
| Parissa Jonoubi
Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
| Majid Ghorbani nohooji
Institute of Medicinal Plants, ACECR, Medicinal Plants Research Center, Karaj, Iran
| Halimeh Hassanpour
Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran
| Atefeh Ashourisheikhi
Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
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| نشانی اینترنتی |
http://nbr.khu.ac.ir/browse.php?a_code=A-10-20-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
علوم گیاهی |
| نوع مقاله منتشر شده |
مقاله پژوهشی |
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