People face breaks in their daily tasks that effect on their daily life. The present study was designed to evaluate the effect of different break activities on eye-hand coordination in female students. In the current experimental study conducted with repeated measures design, 36 high school female students with age range 13-15 years old were conveniently selected. In order to evaluate participant's mental activities prior, the main task, daily distraction of them was assessed using a mind wandering questionnaire and visual analogue scale and measuring eye-hand coordination was done by mirror tracking task. Data were analyzed by SPSS software at significant level P≤0/05. The results showed there was significant difference in eye-hand coordination task after break activities (P<0/05). Pairwise comparisons showed a significant difference in the mirror tracking task performance after music-open eye rest and music-active video game break which showed eye-hand coordination after music was better than other breaks. Present findings show that different breaks have different effect on eye-hand coordination performance in female students and listen to music is the best break activity for them on this task.