The purpose of the current study was to determine the effect of sport vision training on vision perception and decision making of handball referees. In this semi-experimental study, 30 handball referees were Participated among all the referees working in the Iranian Handball Premier League, then the selected referees divided into 2 sport vision training and control groups, randomly. The study included pre-test and post-test phases. Subsequently, participants completed Frastig vision perception test and made decisions after watching 30 prepared clips in pre-test phase. Afterwards, participants completed performing 8 weeks of determined sport vision training, each week included 3 sessions and each session lasted 45 minutes. Henceforth, the participants re-performed Frastig vision perception test and repeated decision making process after watching 30 determined clips. Finally, the collected data were analyzed by paired sample t-test and covariate statistical methods. The results indicated that sport vision training and decision making training significantly affected vision perception and decision making of handball referees. Therefore, the results suggest the utilization of vision perception and decision making training as an effective method in order to improve decision making and vision perception.