This study aimed to assess the effects of acute calcium lactate supplementation intake on the performance and buffering capacity of elite amateur boxing athletes. 8 elite amateur boxers (180±4.33 cm, 24.12±3.68 years, Weight categories: -75, -64, -69, +81) were assigned to acutely receive calcium lactate (0.5 g, 80 mg calcium lactate/kg body weight) and placebo in a double-blind crossover design 90 minutes before the box competition. 90 minutes before, before competition and immediately after, blood samples were taken and lactate, PH, HCO₃^-, and PCO₂ and also, average heart rate, and number of effective hand kick were evaluated. lactate and HCO₃^- significantly increased and decreased at three evaluation times in calcium lactate supplementation and placebo groups respectively (P<0.05). There was no significant difference between calcium lactate supplementation and placebo groups in lactate, pH, HCO₃^-, PCO₂, heart rate and performance at three evaluation times (P>0.05) .Calcium lactate supplementation improves performance without significant changes in the buffering system. In other words, more effort with a similar acidity to placebo could indicate the effect of calcium lactate supplementation on the performance of amateur boxing athletes.