A revision was carried out on the genus Lappula Moench (Boraginaceae) in Khorassan provinces. A total of herbarium specimens collected from different parts of the studied area, including the three provinces of North, Razavi and South Khorassan, were studied. All specimens are deposited in Ferdowsi University of Mashhad Herbarium, (FUMH). According to the results of the study, there are 6 Lappula species in the region: L. barbata, L. microcarpa, L. semiglabra, L. sessiliflora, L. sinaica and L. spinocarpos. In addition, by revising the annual specimens that had been previously misidentified as L. drobovii, a perennial species, these specimens were identified as L. sessiliflora. Identification key, distribution map and species pictures in this article have been presented.