The purpose of this study was to investigate how Iranian experts and adolescents define the 24 character strengths. With this aim in mind, through on purpose method of sampling, semi-structured interviews about definitions of the 24 character strengths were conducted with ten specialists (including psychologists, counselors, and teachers). Furthermore, the opinions of two gender-segregated groups of nine grade students about character strengths’ definitions were discussed in focus groups for girls (N= 7) and for boys (N= 6). Students were selected based on their availability at the time of research and their willingness to take part in discussion groups. Data were analyzed using content analysis and directive approach. Results showed that there was conformity with Peterson and Seligman’s (2004) definitions and Iranian experts-adolescents’ definitions in twelve character strengths and also there were some differences in definitions of other character strengths (e.g., bravery, zest, and honesty). These similarities and differences were discussed. Based on the findings of this research, it can be claimed that there is almost compliance between definitions of the 24 character strengths in Iranian society and the definitions of the character strengths and virtues classification and this classification can be used in research related to this topic.