Objective: The aim of this study was to investigate the effect of non- stability surface of support leg on the instep kicking kinematics in soccer players. Materials and Methods: 30 male soccer players participated in this study. After the measurement of static and dynamic balance tests by the Biodex System, 20 players were selected who were at a desired level of the balance. Data were recorded using the three- dimensional motion analysis system with 6 optoelectronic cameras (200 HZ). The kinematic parameters in three critical moments of kicking (Forward swing of hip, Contact to ball, Follow through) were compared by using repeated measures of variance and independent t- test (0/05). Results: The results showed that the values of maximum angular velocity and displacement of hip and velocity of ball at the kicking over stability surface was significantly higher from the kicking over non- stability surface. Conclusion: These results indicate that the kicking over non- stability surface causes the lower of kinematic parameters in the more of the kicking skill phases and the movement prime velocity of ball