The aim of present study was comparison of advanced physical fitness profile between Olympic soccer team members of Iran according to playing position. 29 Invited soccer players to the 17th national Olympic soccer team of Iran dispatched to 17th Asian Olympics Games, Incheon 2014 )mean age of 20.39± 2.01 yrs(, divided into five group of goalkeepers, defenders, midfield players, wingers and attackers according to playing position participated in the study. Body composition parameters (including body mass index, body fat percentage), isokinetic force (peak torque, agonist/antagonist ratio, work fatigue, total work of extension and flexion), aerobic power by analysis of respiratory gases and anaerobic power and fatigue index by Wingate test were packaged for data collection. Descriptive statistics (i.e., mean ± standard deviation) and inferential statistics (i.e., one way ANOVA with Hopkins post hoc after subtracting data  of pre- post) used for analysis data. While it was unbelievable the findings did not show any significant difference between five groups of present study. It seems that Olympic soccer team players of Iran has not prepared in a conditioning principle and based on playing position for participate in 17^th Asian games. Moreover it supposed that national players spent most of the preparation periods & specific based conditioning workouts on their clubs, hence due to the short time of conditioning in the national camps trainings, players have not dispatched to the games with favorable preparation.