Eotaxin, a pro-inflammatory Adipokine is secreted from adipose tissue, which its secretion increases in the obesity and cause neurodegeneration. BDNF is a neurological factor that interferes with cognitive function, fat and sugar metabolism, as well as the pathophysiology of obesity and metabolic syndrome in adulthood. The aim of this study was to investigate the Effect of Six Weeks of Interval Resistance Training on Eotaxin and Brain-Derived Neurotrophic Factor Serum Levels in Overweight young men. Twenty non-active students without regular exercise program from Kharazmi University were randomly selected and divided into two groups of training (n=10) and control (n=10). The training group performed Interval resistance Training for 6 weeks, 3 sessions per week and each session lasted 45 minutes. The training program included 7 moves with intensity of 30-40% of 1RM and velocity 2v for 10 seconds and rest intervals with intensity 30-40% 1RM and velocity v for 20 seconds. Blood samples were taken before and after 6 weeks of training to evaluate the serum Eotaxin and BDNF levels using the Sandwich ELISA. To analyze the data, the covariance test and Bonferronchr('39')s post hoc test were used to compare the variables. The results of covariance analysis and Bonferroni test showed that 6 weeks of Interval resistance training caused a significant reduction in the weight (p=0.003), body mass index (p=0.004) Waist circumference (p=0.012) and fat percentage (p=0.001). Also, significant increase was observed in muscular endurance (p=0.001), serum BDNF levels in the training group compared to the control group (p=0.024), and serum Eotaxin levels in the training group decreased significantly (p=0.022). Correlation results showed a significant reverse relationship respectively between pre and post training Eotaxin and BDNF (p=0.015   r=-0.511, p=0.013 r=-0.586). Weight loss through six weeks of Interval Resistance training, which accompanied the loss of adipose tissue; resulted in a reduction in serum levels of Eotaxin and increased serum levels of BDNF.