The aquaporin5 (AQP5) is an integral membrane protein that plays an important role in maintaining water homeostasis in the lung during of puberty. The purpose of this study was to evaluate the effect of period of reduced training with Nigella Sativa (NS) supplementation on AQP5 levels in the lung of Male Wistar rats during of puberty. 110 newborn three-week old male Wistar rats with average weight of 68±9 gr, were randomly divided into three groups included; the base, control and training groups. After a 6-week training period, the control group was divided into 2 groups with and without NS and training groups were divided into 6 groups including frequency, volume and intensity taper with and without NS. After 6 weeks, NS supplements were fed daily by gavages to groups of NS 500 mg/kg body weight. Progressive interval training was performed in 6 sessions, 30 minutes each session at a speed of 15 to70 meters per minute, Then, taper phase for three weeks in decreasing pattern, intensity, frequency and volume were performed. AQP5 levels were measured by ELISA methods. Analyzed is done with two-way ANOVA and LSD test at α≤0.05. While NS supplementation were increase average AQP5 levels in the taper groups of frequency, volume and intensity, But, there were no significant differences (P≥0/45) between AQP5 levels in with and without NS groups. Furthermore, three-week taper versus two weeks taper a significant increase the average AQP5 levels in the groups of with and without NS. It is suggested that the antihistaminic and antinociceptive effects of the NS with a period of reduced training with increasing levels of AQP5 would improve the physiological and functional parameters of the lung during of puberty.