Osteoporosis is characterized by low bone mineral density resulting into an increased risk of fracture (9). The aim of the study is determine the effect of aerobic exercise with blood flow restriction (BFR) and vitamin D supplementation on ostosis metabolic markers (OMM) among middle aged females. In this study 30 females with age of 42.76±7.44, height of 157.90±5.74, weight of 70.55±9.68 and BMI of 28.33±3.87, divided in three groups of 10 subjects, randomly. Two empirical Groups with and without BFR, both with vitamin D supplementation, one hour before training, performed aerobic exercise during eight weeks, three days a week, one hour a day, with 50 - 70% intensity of maximal heart rate. Density of OMM, calcium, phosphorous and alkaline phosphate examined in lab, 48 hours before and after training period in saving status before breakfast. Aerobic power of empirical groups determined with 12 minutes run/ walking cooper test. For data analysis used descriptive statistics, ANOVA and analysis variance with repeated measures. Results showed that eight weeks exercise with BFR and vitamin D supplementation, the density of calcium and phosphorous increased significantly. The density of Alkaline - phosphate did not show variation significantly. In the other hand, only the rate of calcium showed variation significantly, which increased in without BFR more than others. In conclusion, aerobic exercise with and without BFR and vitamin D supplementation can increase the density of OMM, calcium, phosphorous and alkaline- phosphate. But only the density of calcium showed increasing significantly.