The purpose of this research was to determine the acute effects of plyometric training with changing of height landing on the electromyographic activity during preparatory phase land-jump in male students. The population of this research were 10 physical education male students of Kharazmi University, which selected as sample. (21/10±1/286yrs, 173±6/467 height, 68/2±7/42 weight). In four different days subjects Performed as single leg one of four protocols plyometric training and land - jump from a height of 40 and 60 cm. EMG activity of the quadriceps and hamstring muscle group 150 milliseconds before foot contact with the ground was recorded. Analysis of variance with repeated measures and Bonferroni post hoc test was used. The results of this study showed that muscle activity after changing of height landing in preparatory phase (from 40 to 60 cm) has increased significantly (P=0/002, P=0/001, P=0/001) respectively. Activity of muscles (rectus femoris, vastus lateralis, biceps femoris) during landing after performing plyometric and changing of height landing in preparatory phase has increased significantly (P=0/039, P=0/001) (P=0/006, P=0/000) (P=0/001, P=0/014) respectively. Jump height has increased significantly (P=0/007, P=0/002) respectively. The results showed that acute perform plyometric training with changing of height landing result in significant changes in the electrical activity of muscles and also the height of the jump in the preparatory phase.